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Literature summary extracted from

  • Nguyen, H.H.T.; Elliott, S.J.; Yip, J.H.K.; Chan, S.I.
    The particulate methane monooxygenase from Methylococcus capsulatus (Bath) is a novel copper-containing three-subunit enzyme. Isolation and characterization (1998), J. Biol. Chem., 273, 7957-7966.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.18.3 genetic analysis of pMMO genes Methylococcus capsulatus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.18.3 additional information native occurence of a truncated form of pMMO with different molecular weight Methylococcus capsulatus

General Stability

EC Number General Stability Organism
1.14.18.3 pMMO is very unstable in vitro Methylococcus capsulatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.3 membrane membrane-bound particulate enzyme form termed pMMO Methylococcus capsulatus 16020
-
1.14.18.3 membrane enzyme from cells grown under conditions of high copper availability is membrane-bound Methylococcus capsulatus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.3 Cu+ pMMO, requirement for, contains 12-15 Cu+ ions per molecule of enzyme Methylococcus capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.3 23000
-
 1 * 45000 + 1 * 26000 + 1 * 23000, pMMO, SDS-PAGE Methylococcus capsulatus
1.14.18.3 26000
-
 1 * 45000 + 1 * 26000 + 1 * 23000, pMMO, SDS-PAGE Methylococcus capsulatus
1.14.18.3 45000
-
 1 * 45000 + 1 * 26000 + 1 * 23000, pMMO, SDS-PAGE Methylococcus capsulatus
1.14.18.3 94000
-
 pMMO Methylococcus capsulatus

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.3 Methylococcus capsulatus
-
 Bath
-
1.14.18.3 Methylococcus capsulatus
-
 enzyme form pMMO is copper-inducible
-
1.14.18.3 Methylococcus capsulatus Bath
-
 Bath
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.14.18.3 sensitive to O2, 3 kinetically distinct froms of pMMO with respect to O2 tension, type I is stable with moderate activity, type II is highly unstable to oxygen, type III is an intermediate form Methylococcus capsulatus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.3 pMMO after induction with copper, kinetic type I with respect to O2-sensitivity Methylococcus capsulatus

Storage Stability

EC Number Storage Stability Organism
1.14.18.3 -80°C, pMMO kinetically type I with respect to O2-sensitivity, repeated freeze-thaw-cycles, stable Methylococcus capsulatus

Subunits

EC Number Subunits Comment Organism
1.14.18.3 trimer 1 * 45000 + 1 * 26000 + 1 * 23000, pMMO, SDS-PAGE Methylococcus capsulatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.18.3 6.8
-
 or below: pMMO is irreversibly inactivated Methylococcus capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.3 NADH
-
 Methylococcus capsulatus